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The labelling pattern with H-TdR has been studied on in vivo labelled imprints and histological sections.
Here, we present a novel and unique tool, mzMatch ISO, that circumvents these bottlenecks by performing fully automated targeted or untargeted annotation and relative quantification of mono-isotopic and corresponding isotope-labelled peaks of metabolites in stable isotope-labelled LC MS data to generate plots and tables that describe the labelling pattern in detail.
Both cytoplasmic localization and the plasma membrane labelling pattern suggested that sialin may possess multiple transport functions in different cell types.
Furthermore, the isotopic labelling pattern of ethene provided the first direct experimental evidence for ethene formation by a paring-type reaction from polymethylated benzene intermediates.
Comparison of the labelling pattern of pyruvate and acetyl-CoA showed that the 13C-content of pyruvate was higher than the corresponding labelling of acetyl-CoA, indicating that a less labelled source was contributing to the acetyl-CoA pool.
Colloidal gold particles were restricted to endothelial cells, showing an asymmetric labelling pattern, which was always characterized by markedly higher density of immunolabelling of the abluminal rather than the luminal plasmalemma.
This labelling pattern overlapped that of gata3 at the same stage [17].
A fiber-like labelling pattern is also observed for the H3K20me3 mark.
Immunohistochemistry conducted in cerebellar tissue from unc5c −/− and +/+ mice revealed no differences in the labelling pattern (Fig. S1D).
Background was subtracted and brightness/contrast were adjusted in the images to render an all or nothing labelling pattern using Corel Draw version 12 (Corel, Ottawa, Ontario, Canada).
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The labelling pattern of succinate that would have originated from the glyoxylate cycle was calculated from Asp and Lys fragmentomers representing the labelling patterns of Oaacyt and AcCoAcyt respectively.
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