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Herman LW, Fischman AJ, Tompkins RG, Hanson RN, Byon C, Strauss HW, Elmaleh DR. The use of pentafluorophenyl derivatives for the 18F labelling of proteins.
The results have important consequences for the labelling of proteins, specific immobilisation, and production of novel modified proteins for pathophysiology in diseases involving oxidative dysfunction [2].
Phosphorescent metal complexes that bind and interact with proteins in a non-covalent fashion are also discussed as is the use of metal complexes as single-electron transfer photo-catalysts for the fluorescent labelling of proteins.
The process was adopted from a sensitive immunogold-silver staining procedure, whereby the secondary colloidal silver deposition step improved the labelling of proteins (Champod et al., 2004).
We have shown that the THP bifunctional chelator is potentially a solution to the problem that labelling of proteins with gallium-68 typically entails harsh conditions such as high temperature and acidic pH, as well as post-labelling purification, thus opening the door to kit-based protein labelling with gallium-68.
Among various systems to produce heterologous proteins for structural and functional studies, protein expression in Escherichia coli is the most commonly used system because it is genetically simple, inexpensive for producing large quantities of proteins and permits the isotopic or heavy atom labelling of proteins that is necessary for some structural methods.
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DIGE involves fluorescent labelling of protein mixtures with Cy-dyes in order to determine relative differences in protein expression.
Recently, histone acetylation was studied using metabolic labeling of proteins with heavy 13C-labeled acetyl-CoA produced from 13C-glucose in human cells and analysis by mass spectrometry (MS 7.
Liu, D., Xu, R. & Cowburn, D. Segmental isotopic labeling of proteins for nuclear magnetic resonance.
Guo, C., Geng, C. & Tugarinov, V. Selective backbone labeling of proteins using 1,2-13C2-pyruvate as carbon source.
Cysteines with reactive thiol groups are attractive tools for site-specific labeling of proteins.
More suggestions(15)
tagging of proteins
evidence of proteins
tag of proteins
packing of proteins
labels of proteins
naming of proteins
labelling of sodas
labelling of cigarettes
labelling of biomolecules
labelling of bacteria
labelling of bisphosphonates
labelling of genes
labelling of sequences
labelling of others
labelling of mixtures
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