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Hek293 cells that did not express GHR showed no Cy3-GH labelling (not shown), indicating that the entire fluorescent label originated from GHR activity.
The rabbit anti-pE-Aβ and mouse anti-pE-Aβ antibodies showed an identical staining pattern in mouse and human brain tissue as revealed by double immunofluorescent labelling (not shown).
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The cerebellum presented labeling in Purkinje cells as well as abundant microglia labeling (not shown).
This protein was made to maintain an intact C-terminal –GCUG motif and the incorporation of selenocysteine was verified by 75Se labeling (not shown).
The majority (>90%) of these mRNAs localized to U-87 astrocytoma RISC are associated with tumorigenesis and cancer (label not shown on biological pathway to avoid figure crowding), which suggests the miRNA-loaded RISC is a key regulator of mRNAs involved in cancer and cell cycle regulation in U-87 astrocytoma cells.
This application resulted in transduction of 70 75% of nigral DA neurons as judged by EGFP/TH co-labelling (not shown).
DA1 and VA1d glomeruli are outlined together (white dashed line) and were determined using the accompanying mtdT label (not shown).
BV-G2/p17 virions were never seen associated with gold grains, and most immunogold labeling was found at distance from BV particles, and corresponded to nonspecific, background labeling (not shown).
Dact2 showed prominent expression in young somites and the developing intestine; in more strongly stained specimen, all somites as well as the trigeminal, facial and glossopharyngeal ganglia were labeled (not shown).
In sections processed to visualize the mRNA for the vesicular glutamate transporter 1 (VGLUT1), all tectal nuclei (including the TLCd, TLCv, and SC) are completely devoid of labeling (not shown).
At day 9, 2.8% ± 1.8 of cells still retained their labels; however, all cells eventually lost their labels (not shown), indicating that the monolayer culture conditions are incompatible with long-term cellular quiescence and that all cells divide, although some are slower than others.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com