The amino-allyl indirect labelling method was used to obtain Cy3 (reference) or Cy5 (samples -labelled cDNA.
Briefly, total RNA was extracted from dissected gill samples and the amino-allyl indirect labelling method was used to obtain Cy3 (reference) or Cy5 (samples -labelled cDNA.
To minimise dye-label incorporation differences, aminoallyl labelling has been used, which results in higher labelling efficiency and improved incorporation of the different dyes compared to the direct labelling method [ 6- 8].
The indirect labelling method was of the highest specificity.
Developing an easy, rapid and performant labelling method is important.
For the J591C diabody, this labelling method proved robust, allowing for reproducible labelling to high specific activity.
Cells were radiolabelled, WBCs using modified (incubation time 20 min) EANM recommended 111In-oxine WBCs labelling method.
However, the PP protocols are still simple and routine such that there remains substantial benefits to a very simple, fast and reliable labelling method.
The spin labelling method has been used in a large variety of situations, in the broad field of polymers at solid interfaces.
There are a wide range of labels and methods for attaching these to polymers, and the resulting fluorescence behaviour can depend on both the label and labelling method.
The spin labelling method is exemplified using studies of pharmaceutical systems designed to prevent skin blistering, and spin trapping is illustrated with studies of oxygen-centred radicals.
