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When using HyPer5-dCTP for labelling, increasing the amount of dye in the reaction will increase yield of labelled targets desired for hybridization.
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It could be demonstrated that uncontrolled labelling increased protein spot volume, even doubling it in some cases.
The punctuate labelling increased during differentiation (b, enlarged in b'; 6-day differentiation) and was lost upon competition with the DUX4c peptide (c).
PLIN-2 labelling increased as well in lipid-loaded cells compared with control cells.
Labelling increased with concentration of 2 up to 25 μM, above which solubility in cell culture medium became limiting.
Similar to the paleocortex, plasma membrane labelling was dominant in the superficial layer at P3 (69%%) and with maturation the transport vesicle-associated labelling increased (P3: 31 %; P12: 41.8 %).
Plasma l-[ring-C6]phenylalanine labelling increased over time and remained stable during the last 3 h of post-exercise recovery (Fig. 2).
Muscle tissue free l-[ring-C6]phenylalanine labelling increased over time, averaging 0.046 ± 0.002 and 0.061 ± 0.001 (TTR) in the biopsy samples taken immediately after exercise and after 6 h of post-exercise recovery, respectively (P < 0.05).
In contrast, Jim13 labelling increased in the whole stigmatoid tissue (Fig. 6C), with higher secretion accumulation outside the cell walls, both in the stigmatoid tissue and on the papillae (Fig. 6D).
Compared to control, UCP2 protein labelling increased moderately in the I/R (+39%, NS) and IPC+I/R (+28%, NS) groups, and substantially in the IPC group (+339%, P < 0.05).
Other research by Dr. Wansink found that descriptive menu labels increased sales by as much as 27percentt.
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