The percentage of positively labelled cells (0 100%) in each section was multiplied by the intensity of labelling, graded from 0 (no labelling) to 3 (intense labelling), providing a score between 0 and 300.
First, it should be labeled "Grade 1" or "Grade 2".
The "grade F meat" joke was written by Mirkin, inspired by his cousin once seeing a box of hot dogs labeled "grade C, approved for human consumption".
The data in each file were reduced to high-quality probe sets attributable to single, full-length mRNAs (labeled "grade A" in Affymetrix annotation files; Mouse430_2.na32.annot.csv and HG_U133_Plus_2.na32.annot.csv).
The DNA probes were random primed, labeled with Digoxigenin-11-dUTP using DIG-High Prime, a 5 × concentrated labeling mixture of random hexamers, dNTP mix containing alkali-labile Digoxigenin-11-dUTP, and labeling grade Klenow enzyme, and optimized according to the manufacturer's manual (Roche; DIG-High Prime DNA Labeling and Detection Starter Kit II).
The culture was split into two flasks and methionine was added to a final concentration of 60 ng/ml, of which 0.5 ng/ml was [S] methionine (cell-labelling grade 1175 Ci/mmol; New England Nuclear, Boston, MA).
The colander separates the very highest quality Grade A beans from those labeled Grade B; the two grades are sent separately down the hill in a water chute with a 1 percent gradient.
Britain came close to adopting a traffic-light system of compulsory food labelling to grade the health impact of food products - but, as Jacques Peretti reports, opposition from within the food industry prevented it happening.
Both of these markers demonstrated a nonsignificant trend towards higher labelling with grade.
The fragment was labelled with P-dCTP (Amersham), using a random hexanucleotide mix and labelling-grade Kleenow enzyme (Boeringer-Mannheim). Stringency washes were performed at 65°C for 2 × 5 min in 2 × SSC, 0.5% SDS and for 4 × 5 min in 0.2 × SSC, 0.1% SDS.
