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Confocal microscopy analysis (0.25 µm steps along the z-axis) of double labelling experiments on the mature organ of Corti showed a colocalization of vezatin and radixin at the same emplacement as β-catenin and p120-catenin along the OHC DC TAJs.
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A first attempt to demonstrate such hypothesis was tentatively proved by DiI labeling experiments on amphioxus, showing that ventrally-located cells migrate toward dorso-lateral sides of the embryo [ 59].
To validate the domain localisation within the complex, we have employed another approach based on labelling experiments using nanobodies (NBs).
Finally, this chapter concludes that the advances in multi-fluorescent labeled experiments depend on the implementation of newly engineered reporters and experiments that exploit the power of direct visualization.
To determine the effect of mutant Comp expression on chondrocyte proliferation, we used 2 hr BrdU labeling experiments on 3-week-old mice.
Our revision includes the suggested Brdu labeling experiments on samples at informative time points challenged as in original Figure S3 (now Figure 4 figure supplement 3).
SF performed replication pulse labeling experiments.
Our BrdU-labelling experiments on onychophoran embryos revealed specific labelling patterns corresponding to some developing structures and organs.
Firstly, combinations of double-labelling experiments on CHO cells, thanks to the lipid analogs bearing the red spectral BODIPY we had synthesized, showed excellent co-localization of BODIPY505-LacCer with BODIPY589-GlcCer, but almost complete segregation from BODIPY589-SM ([31], Fig. 8).
The glyoxylate cycle is under glucose repression [ 34] and no in vivo activity of the glyoxylate cycle in S. cerevisiae was previously observed in the C-labelling experiments on glucose either under fully aerobic conditions or in 2.8% oxygenation [ 23].
The reported NGAM of P. pastoris was 2.26 mmolATP/gDCW h (Chung et al. 2010), while, according to our calculation based on 13C labeled experiment, the NGAM was 9.48 mmolATP/gDCW h for P. pastoris G1HL (Nie et al. 2014).
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