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Besides the chemical entity of the targets both protocols differ with respect to preparation steps such as fragmentation (chemical versus enzymatic), labelling ("during isothermal amplification" versus "after fragmentation") and the position of the label (throughout the sequence versus end-labelled).
At this stage in the protocol the dye should be silenced; if it is not, unspecific labelling during sample pooling would occur in DIGE.
To perform systematic clonal analysis in the developing mouse embryo, we applied a method that relies on the use of ubiquitously expressed knock-in alleles and is suitable for non-invasive permanent cell labelling during embryonic development [10].
To test if the loss of iridophores is caused by apoptosis, we used TUNEL and acridine orange labelling during larval development to detect dying cells.
LRC can be identified in the murine mammary gland following labelling during the period of ductal morphogenesis in the neonate [ 21, 31, 32].
Figure 3a is a representative bi-variate contour plot at 5 hours after pulse labelling during exponential phase illustrating cells in G1 and G2/M phase as well as the undivided (flu) and divided (fld) fractions.
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In contrast, the pools of both G6P and sucrose were rapidly labelled during the pulse and de-labelled during the chase.
Dukovic began photographing the label during the spring, 2013, season.
The result was Song to the Siren, which appeared as a white label during the summer of 1992.
It was borrowed, Ms. Catliff said, from Ms. Hindmarch's showroom in London, to promote the label during Fashion Week.
Also at the Jazz Standard, Greg Osby fills the room with up-and-comers from his Inner Circle Music record label during a three-day mini-festival.
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