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Cortical areas that yielded a response on testing were labelled with small numbered paper tickets.
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In a subsequent step, ncAAs are labeled with small dyes via click-chemistry.
This stretch of six amino acids can be labeled with small dyes such as FlAsH (Adams et al., 2002).
In most of these studies, biomolecules of interest are labeled with small fluorescent dyes to report their localizations and dynamics.
Antibodies in particular are often labeled with small fluorophores instead of genetic tags due to the complexity of adding fluorescent proteins to the multichain immunoglobin molecule.
The N-termini of Aβ peptides are easily labeled with small, organic fluorophores including fluorescein, rhodamine and HiLyte based dyes, and these N-terminal labels do not significantly perturb the peptides.
For most of the single-molecule fluorescence studies to date, biomolecules of interest are labeled with small organic dyes which suffer from their limited photostability evidenced by blinking and photobleaching.
The techniques used are similar to those described above for polyQ repeats with an added emphasis on labeling with small fluorophores, particularly for the short Aβ peptides, and a number of studies using luciferase, or FP complementation to study oligomerization and aggregation.
Figure 1 shows a segment of the cholesterol biosynthesis pathway labelled with abbreviated small molecule names; Figure 2 shows full names.
Single-molecule FRET is basically a 'molecular ruler' that can measure the distance between two different molecules if each is labelled with a small dye molecule that emits fluorescent light.
In an effort to provide additional structural insight into the organization, stability and regulation of GPCR oligomers by ligands, we used FRET to study oligomerization of purified β2AR site specifically labelled with relatively small fluorescent probes and reconstituted into a model lipid bilayer.
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