The hydrophilicity of the Ga-labelled monomer and dimer labelled with either Ga or In was not different.
Probes directed against CDK4 (RPDGFRAN19,RP11-231C18, PDGFRP11-117E831C18, RPTEN626H4, RP11-105A10, and PTEN (RP11-105A10) were labelled with Cy5 (GE Healthcare, Amersham, UK), whilst commercial probes against RB1 (Abbot Molecular) and CDKN2A/B (Abbott Molecular) were SpectrumOrange-labelled. Appropriate centromeric probes were labelled with either fluorescein or SpectrumGreen.
The synthesized cDNA was transcribed into cRNA and labelled with either cyanine 3 or cyanine 5-labelled nucleotide (Perkin Elmer, Wellesley, MA).
Purified genomic DNA of all isolates was labelled with either Cy3- or Cy5-dCTP, pooled with labelled DNA of the reference Tohama I strain, and hybridized on pre-treated slides.
Matched pairs of particles, labelled with either donor (phenanthrene) or acceptor (anthracene) groups, were prepared by three-stage emulsion polymerization under monomer-starved conditions in which varying amounts of methacrylic acid were introduced into the third-stage polymerization.
Estimation of the optimal therapy setting has been performed in this study for preclinical testing of PP-F11, assumed to be labelled with either 90Y, 177Lu or 213Bi.
Nuclei were labelled with either chromomycin A3 (Sigma) or TO-PRO3 (Molecular Probes).
PCR fragments were fluorescently labelled with either 6-FAM, NED, PET or VIC fluorescent dyes (Applied Biosystems).
The 5' end of each forward primer was fluorescently labelled with either Hex, Fam (MWG Biotech, Germany) or Ned (Applied Biosystems, UK).
The cDNA was then transcribed into antisense cRNA and labelled with either CTP-Cy3 or CTP-Cy5 fluorescent dyes for 2 h at 40°C following the manufacturer's protocol.
Each of 3 replicates had one N form labelled with either ca.4 kBq 14C (peptide and amino acids) or 98 atom % 15N (NH4+ and NO3-; Sigma Aldrich, Gillingham, UK).
