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Following amplification and purification, 300 ng of each Cy3 labelled test and 300 ng of Cy5 labelled reference pool cRNA were mixed together and incubated with Fragmentation and Hybridization buffers according to manufacturer's instructions.
Experimental results show that with the utilization of these newly labelled test cases, the effectiveness of fault localization can indeed be improved.
In this technique, differentially labelled test DNAs from a tumour and from normal tissue are co-hybridised to metaphase chromosomes [ 26].
The MAQ technique consists of the quantification of fluorescently labelled test and control amplicons, obtained by a single multiplex PCR (mPCR) amplification.
The differentially labelled test and reference probes were then combined in the presence of canine Cot-1 competitor DNA, ethanol-precipitated and resuspended in hybridisation buffer as described by Dunn et al (2000).
Aliquots of 400 ng labelled test DNA and control DNA were ethanol precipitated with 50 μg unlabelled human Cot1 DNA (Life Technologies, Breda, the Netherlands) and 10 μg of salmon sperm DNA (Sigma, Zwijndrecht, the Netherlands).
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Thereafter, these action classes are used to label test images.
This test, however, requires [14C] labeled test substances, which clearly limits the scope of the test.
The formulae synthesize the counts of positively labeled test storylines for each band.
For instance, the transition weights could be learned from a labeled test set, or a more complex tracking method like the high-order HMM scheme from Chang et al. [38] could be used instead.
1 ml from this was then transferred to 9 ml of the 10−2 labeled test tube i.e. 10−2 dilution, using a fresh sterile pipette; and this was repeated for each succeeding step till 10−5.
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