Sentence examples for labelled template from inspiring English sources

To produce labelled template for hybridisations aRNA was generated using a MessageAMP II aRNA Amplification Kit (Ambion) as per the manufacturer's instructions.

Briefly 825 ng cDNA of each labelled template was fragmented in the dark and made up to a final volume of 20 μl with nuclease free dH2O.

Prior to hybridisation, 825 ng of each labelled template was fragmented in the presence of 11 μl of 10X blocking agent, 2.2 μl of 25X Fragmentation buffer (Agilent), and made up to a final volume of 20 μl with nuclease-free dH2O.

The lipophilicity of the compounds was found to depend on the nature of the labeled template and the halogen.

The images were segmented into 90 anatomical regions of interests (ROIs) (45 ROIs for each hemisphere, Table 1) using the anatomically labeled template reported in previous studies [5], [11], [41].

The concentration of the 5′-FAM fluorescently labeled template RNA was determined from the absorbance at 260 nm using the extinction coefficient ε260 = 148 600 M–148m–148

Clusters showing significant between-group differences were then described in terms of their peak coordinates, and the automated anatomical labelling template image regions contained therein.

Of course, it is entirely possible that asymmetric division with preservation of the labeled template DNA strand contributes to gland homeostasis by the quiescent stem cells deposited behind the active growth front.

Although controversial at first, the Cairns model has now been confirmed by the demonstration of asymmetric chromatid segregation using a technique to label template strands of DNA during development with tritiated thymidine and newly synthesized strands with bromodeoxyuridine [ 18].

The catcher is an artificial fluorescently labeled template; it is designed in such a way that the tagging portion of "the pitcher", which previously recognized and hybridized with the target sequence, hybridizes with the catcher.

The principle of FP derives from the fact that the degree of polarization of a fluorophore (in this case FAM) is inversely related to its molecular rotation as indicated in Scheme 2. As shown in Fig. 4, at low concentrations of nanoparticles (0.78 and 1.56 μg/mL), FP of Myr-NQA-MINs after incubation with FAM labeled template was significantly higher than FP of NQA-MINs and NINs.