Exact(1)
However, replacing pyridine with other heterocycles in the GP reagent does not significantly affect the performance of the reagent, giving the potential for future synthesis of multiplexed series of isotopically labelled tags.
Similar(59)
This could lead to the development of what is labelled "tag clouds" and a landscape "folksonomy".
Redox properties of metal based nanoparticles, surface impedance change and conductance changes once nanoparticles are present as labelling tags or modifiers of transducer surfaces are also improving the technology.
One example of this approach is DiLeu labeling tags developed by the Li laboratory.
According to studies in humans and animals, oral administration of labeled PC leads to the rapid appearance of labeled PC in plasma, with rather weak formation of labeled TAGs [ 63].
One experimental shortcoming in measuring a protein's half-life with fluorescently labeled tags is the presence of bulky, folded entities at either the N-, or the C-terminus of the investigated polypeptide.
Labeling: Tagging an actor with a descriptive term that carries a connotation of moral deficiency or social inferiority.
Expression of soluble peanut DGAT (DGAT3, 42 kDa) in E. coli resulted in high levels of DGAT activity and the formation of labeled TAG [ 12].
Whereas substantial amounts of labeled TAG were formed in the presence of the phospholipid mixture used for the acyl-CoA independent assay (see Materials and methods), only traces of labeled TAG were formed when individual phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phophatidylserine) were used as acyl-donor.
If we neglect some effects such as that secondary structures and cross-hybridization, and there is only specific binding between a given probe and its complimentary allelic strand, and between labeled tag and its corresponding probe, we can deduce that the amount of labeled tag-probe bound to the complementary immobilized allelic sequence is proportional to the fluorescence intensity.
This concept pertains to many quantitative labeling approaches (i.e., ICAT, iTRAQ), where an isotopically labeled tagging reagent is used to impart peptides with a defined mass shift or a particular reporter mass.
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