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A fluorescently labelled structure of the specimen itself might act as a source as well.
To use a labelled structure in the specimen is surely the better choice than invasively bringing a feedback source into the sample which can also destroy the structures in the ROI.
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It is divided into three sections, labelled Structures 4a, 4b and 4c.
The majority of 70 kDa dextran labelled intracellular structures by macrophages were >500 nm in diameter and the formation of these dextran labelled structures was inhibited in the presence of the macropinosome selective inhibitor, amiloride, confirming their identity.
Indeed, motility of the eGFP-ZtRab5 labelled structures was abolished when microtubules were depolymerised with the anti-fungal drug benomyl (Fig. 3F).
Finally, the fluorescence images were then co-registered using Adobe Photoshop software, resulting in a new multi-channel image showing the inter-relationship between the overlapping fluorescently labelled structures.
After the slides were rinsed and incubated with the peroxidase-labeled, polymer-conjugated goat anti-rabbit secondary antibody, the labeled structure was visualized.
By looking at a fluorescently labeled structure one molecule at a time, it is possible to side-step the optical diffraction limit and obtain "super-resolution" images of small nanostructures.
The approach for measurement of kinetoplast and nucleus location could be used to measure the location of any fluorescently labeled structure in the cell.
In effect, this means efficient expression of a volume-filling fluorescent marker and a high signal-to-noise ratio for the labeled structure.
CRF is undirected graphical model for segmenting and labeling structured data [19].
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