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If cloning did prove acceptable to the UK public (a big if at the moment) they did not think meat from the progeny of clones should be labelled separately.
For each hybridization (below) adye-swap was used i.e. each sample to be used in a hybridization was labelled, separately, with each of the two dyes (below).
RNA extracted from paired FHIT-positive and FHIT-negative lesions were amplified, labelled separately with either Cy3- or Cy5-UTP and pooled.
The two populations of RMA cells to be tested were labelled separately with 2 μM PKH26 and PKH67 membrane dyes, according to the manufacturer's instructions.
Two cell populations, labelled separately with the membrane dyes PKH26 and PKH67, was injected i.p. into B6 mice at 1 1 ratio (see Materials and methods).
When untransfected RMA cells were labelled separately with PKH26 and PKH67 and injected at 1 1 ratio, the original 1 1 ratio was observed 48 h later in the peritoneal lavage and after culture in vitro (Fig. 4A).
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Each sample was divided into two parts and labeled separately by Cy5 and Cy3 dyes for microarray.
Based on the mobility of the protein bands on the gel, the different enzyme active bands were labeled separately on each gel from top to bottom.
Left and right lateral ventricles were labeled separately.
Briefly, 50 µg of each protein extract was labeled separately at 0°C in the dark for 30 min with 400 pmoles of the N-hydroxysuccinimide esters of cyanine dyes (Cy3 and Cy5 CyDyes; GE Healthcare).
Control and HGPS samples were labeled separately with CyDy2 or Cy3 fluors.
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