Amplified and labeled cRNA was produced using the Illumina specific Ambion TotalPrep kit (Applied Biosystems Inc., Foster City, CA, USA).
Total RNA was extracted from purified cells, and labeled cRNA was produced using the Affymetrix Two-Cycle amplification kit and used to probe Affymetrix Mouse Genome 420A2.0 gene arrays.
Fluorescently labeled cDNA was produced using a two-step procedure involving cDNA production from target RNA using a reverse transcriptase reaction incorporating aminoallyl-modified deoxynucleotide (aadUTP), followed by the second step involving chemical coupling of fluorescent dye (either Cy3 or Cy5) to the introduced amino moieties of the newly synthesized cDNA.
Isotopically labeled (S) rHIgM12 was produced using Trans35S-Label (MP Biomedicals #51006) and validated for binding to the surface of primary cerebellar granule cell neurons in culture (Warrington et al., 2004).
Hybridization was performed in HS containing 0.05 ng/μl of the appropriate DIG-labeled probe (produced using the DIG RNA labeling kit, Roche) for 48 72 hours at 55°C.
This unlabelled aRNA was subsequently reverse transcribed to double stranded cDNA using the GeneChip® Two-Cycle cDNA Synthesis Kit and biotin labelled aRNA was then produced using the GeneChip® IVT Labelling Kit (Affymetrix, CA, USA).
Prop 37 would have required any food sold in grocery stores to be clearly labeled if it was produced using genetic engineering.
Another evidence is produced using labeled fluorescent dsRNA (see below).
Fluorescent labeled V5PB mRNA was produced using 2 μL of ChromaTide® Alexa Fluor® 546-14-UTP (red fluorescence, λEx: 550 nm, λEm: 570 nm, Invitrogen) during an in vitro transcription reaction.
Selective labeling of IgFLNa19 21(1 C-L2271,N-A2272) was produced using a mixture of labeled (1-C-leucine and N-alanine) and non-labeled amino acids (0.1 g for each per liter M9) (Peterson et al, 2001).
Stoichiometric data on growth, substrate uptake rate, storage formation, and on the cellular composition of R. jostii RHA1 together with mass isotopomer distribution data of the labelled amino acids that were produced using the iMS2Flux software [ 47] were used as model input.
