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He labelled lymphocytes to follow what they did in vivo, and showed that they went through particular blood vessels – I then followed lots of those tracks.
In both scans with in vivo and in vitro labelled lymphocytes, anterior and posterior images clearly visualised the inflamed joints at 1.5 h p.i. and joints showed delineation at 4 to 6 h p.i.
PKH67+ CD8β+ T cells were detectable at low levels in PBMC for 49 days post-transfer, demonstrating the stability of the labelled lymphocytes in vivo.
Labelled lymphocytes and macrophages were counted.
Labelled mixed leucocytes can display higher blood pool activity, especially in the early images, due to the presence of labelled lymphocytes and residual erythrocytes.
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In the present work we have implemented an ex vivo labeling strategy to load B cells with a non-toxic SPIO configuration, previously determined to efficiently label lymphocytes [34], in combination with a non-toxic near infrared (NIR) cell membrane labeling dye [35].
After labelling, lymphocytes were incubated at 37°C in a 5% CO2 incubator for 96 h.
With respect to the double labeled lymphocytes, both the percentage of CD4+CD45RO+ cells as well as the percentage of CD4+CD45RA+ cells is increased in patients with moderate-to-severe psoriasis, in contrast to the absolute decrease of total lymphocytes.
For the first time, we could analyze the motility of individual labeled lymphocytes within the lamina propria of the small intestine using time-lapse, three dimensional imaging (Fig. 8, Online Resource ESM_3.mpg).
Spatiotemporal tracking of transferred DsRed labeled lymphocytes revealed integration into the spleen, but invariably rare transferred immune cells have been observed in the ganglion cell layer, regardless of the time interval since injection.
Uptake of fluorescent labeled lymphocytes into lacrimal glands was reduced several fold in mice treated with LTBR-Ig compared to controls, demonstrating functional consequences of the effects of LTBR-Ig treatment on HEV.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com