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Total RNA was used to generate the Cy5/Cy3 labelled DNA by using a cRNA amplification labelled kit (CapitalBio).
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Southern blot hybridization of genomic DNA was performed using fluorescein-labeled probes (ECL Labeling Kit, Roche).
Labeling kit assay buffer was supplemented with 2% FBS.
Labelling efficiency was determined with a dot blot with control labelled RNA (DIG labelling kit; Roche).
Single-stranded cDNA was fragmented and end-labeled with the GeneChip WT Terminal Labeling Kit (Affymetrix).
The probes were DIG-labeled using a kit (DIG-RNA labeling kit; Roche).
DNA probes were 32P-labelled using the Prime-It II DNA labeling kit (Stratagene).
Biotin-labeled cRNA was prepared using the Enzo BioArray HighYield RNA Transcript labeling kit (Affymetrix).
CyeDye-labelled cDNA was synthesised using a MMLV RNA fluorescent labelling kit (TaKaRa Bio).
Labeled cDNAs were synthesized with the FairPlay cDNA labeling kit (Stratagene, La Jolla, CA).
Both digoxigenin- and fluorescein-labeled antisense RNA probes were generated using an RNA labeling kit (Roche).
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