LULC change was done using postclassification comparison method where Landsat image for each year was classified and labeled independently, and then comparison was made using an overlay procedure (Lu et al. 2004).
In a limited number of replicate experiments (where the same cDNA was labeled independently more than once), Affymetrix present calls were extremely reproducible in terms of signal value, while the actual signal values for the absent calls varied somewhat widely.
The AM-SVM treats each class label independently, and ignores the inter-calss relationships.
In the "self-self" hybridization control, the genomic DNA from the reference S. cerevisiae laboratory strain was labeled independently with Cy3 and Cy5 and then competitively hybridized against itself.
Purified 500 bp DNA fragments from each of the 24 accessions were labeled independently using distinct 6 bp nucleotide multiplex identifiers, followed by pooling prior to library construction for NGS.
Here we use 454 pyrosequencing to discover microsatellites from a pooled library of post oak samples using reduced representational libraries based on restriction site conservation (Maughan et al., 2009), with each sample labeled independently using a unique multiplex identifier barcode.
Each sample was labeled independently with Cy3 or Cy5 using the ULS aRNA fluorescent labeling kit (EA-006, Kreatech, Amsterdam, Netherlands) and hybridized with the Maui Hybridization System (BioMicro Systems, Salt Lake City, UT) to whole genome, oligonucleotide microarrays [ 83] obtained from the Keck Center for Comparative and Functional Genomics (University of Illinois, Urbana-Champaign, USA).
Briefly, for data set 1, aliquots of 100 pmol of each synthetic oligodeoxyribonucleotides target were labeled independently at the 3' end with 0.3 mM GeneChip DNA Labeling Reagent (Affymetrix) using 60 U of Terminal Deoxynucleotidyl Transferase, Recombinant (TdT; Promega, Madison, WI) at 37°C for 1 h.
500 ng of RNA/individual was amplified using the Ambion MessageAmp II aRNA Amplification kit (AM1751, Life Technologies, Grand Island, NY). 5 μg of amplified RNA from each sample were labeled independently with Cy3 and Cy5 dyes using the ULS aRNA fluorescent labeling kit (EA-006, Kreatech, Amesterdam, Netherlands).
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