But in California, proponents of a ballot measure that would require genetically modified foods to be labeled immediately seized on the study as support for their cause.
However, target cells are usually labeled immediately prior to assay, which inadvertently introduces additional assay variations due to differences in target cell conditions and labeling/handling processes.
Reference MAbs to VSV-G (Roche Diagnostics), gp41 (Sand), actinctin (Sigma-Aldrich) were labeled immediately prior to staining using Zenon labeling kits (Invitrogen) following manufacturer's instructions.
Samples were labeled, immediately placed in 95% ethanol, and archived at the Great Lakes Genetics/Genomics Laboratory.
Fixation was followed by two washes in PBS and stored at 4 °C or labeled immediately.
Some fibers were also labeled immediately deep to caudal area 25.
The plasma was divided into 1 mL aliquots, labeled immediately, and stored at −20°C until being analyzed for cytokine concentrations.
After 1 h of internalization prior to labeling with Cy3-conjugated secondary antibodies (see Methods), cells expressing pIN-JHE811 showed diminished surface labeling compared to cells fixed and labeled immediately after anti-HA application i.e. at time 0 h (Figs. 6b panels E, F).
The water samples were appropriately labelled and immediately transported in an ice chest to the laboratory and kept in the refrigerator at − 4 °C prior to analysis.
At each sampling point, 50 ml of milk sample was taken and transferred into sterile screw-capped sampling bottles, securely capped, clearly labelled and immediately transported to the laboratory for analysis under ice (4 °C).
