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We show, using a fluorescently labelled form of the peptide, that it follows the dye-filling route to neuronal cell bodies in the region of the lateral and ventral ganglia.
In their new paper, they expressed an inducible and fluorescently labelled form of IK17, a monoclonal antibody specific for ox-LDL, in HCD-fed zebrafish to enable visualisation of ox-LDL in vivo.
Test compounds that were active as inhibitors at concentrations below 100 μM in this assay (labelled 'prescreen' in Table 1) were then subjected to determination of half-maximal inhibition concentration (IC50) of p53 ubiquitination using essentially the same assay methodology, except that a fluorescently labelled form of ubiquitin was used.
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These data demonstrate that microinjection of fluorescently labelled forms of PI3Kα leads to the production of low levels of PIP3 at the PM of cells maintained in the growth medium, which indicates that Cy3-labelled recombinant PI3Kα remains active when microinjected into adherent cells.
[18F]PBR06, a fluorine-18 labeled form of PBR06, is a promising PET TSPO radioligand originally developed at NIMH. [11C]PBR06, a carbon-11 labeled form of PBR06, was designed and synthesized for the first time.
The bound molecules are detected on autoradiographs by the failure of binding of a radio labeled form of the peptide under study; the probes are of the same dimensions as glass microelectrodes and hence are relatively atraumatic.
To find these patterns, researchers used to expose rats to an odor for 45 minutes--an unnaturally long time--then kill them and look for changes in the uptake by the olfactory bulb of a labeled form of glucose, which indicates neuronal activity.
To investigate gastrointestinal absorption of intact gluten peptides, we dosed a gluten-sensitive macaque with an isotopically labeled form of the immunotoxic 33-mer gluten peptide [13], and measured its plasma concentration using a sensitive and specific mass spectrometric method.
One of these mutants has been used to prepare a fluorescently labeled form of CNTF, a therapeutically important protein.
The latter compound was used to create a fluorescently labeled form of Ciliary Neurotrophic Factor (CNTF), a protein of therapeutic importance.
A labeled form of vitamin B-12 would be the ideal internal standard because it should behave similarly to the unlabeled endogenous form, including binding to the 2 circulating vitamin B-12 binding proteins (haptocorrin and transcobolamin).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com