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For the methyl labelled compound CD3CH2OH, a total exchange was observed during the production of methane from adsorbates, but only partially in the case of ethane.
An alternative mitochondrial directed fluorine-18 labelled compound is F-18 fluorobenzyltriphenlphosphonium (F-FBnTP) (Table 11).
The results obtained with this pretargeting protocol are highly encouraging, particularly in light of the fact that significant survival prolongation could be obtained even when then labelled compound was administered intravenously.
Appropriate amounts of each intermediate stock solution were mixed and, once again, diluted gravimetrically with toluene to obtain calibration working solutions covering the range 50 150 μg/kg of each native compound and containing 100 μg/kg of each isotopically labelled compound.
This is achieved though protein labelling with isotopically distinct tags (ICAT) or through the incorporation of isotopically distinct amino acids (SILAC), or a stable isotope labelled compound which represents the sole source of an element, typically nitrogen or carbon (metabolic label-ling).
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Cellular uptake studies with fluorescently labelled compounds showed that guanidinylated chimeras were taken up four times more efficiently than Tat47 57.
The isotope labelled compounds were used as internal standards (IS) for quantification.
All three different labelled compounds showed very low accumulation in kidneys, comparable to other studies with MG11 [16].
Uptake assays were performed using radioactively labelled compounds, [C]carboplatin, [H]FA, and [H]MTX.
The radioactive band corresponded to the Toluidine Blue stained PGs, indicating that PGs were the main S-sulfate labelled compounds.
Once every 24 h an aliquot was withdrawn to determine the integrity of the labeled compound by means of HPLC.
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