Under these conditions, LG labeled by expression of GFP-FasL were easily observed in close proximity to the PM.
We used a strain in which blood vessels are labeled by expression of kdrl:mCherry (VEGFR2) and flt4 Citrine (VEGFreporterstereporters
Here, the eight dopaminergic neurons of the worm are specifically labelled by GFP expression from the promoter of the C. elegans dopamine transporter.
Using a zebrafish line in which neutrophils are labelled by GFP expression, the team screened a library of fungi-derived compounds and pinpointed a mycotoxin and a natural antibiotic with specific inhibitory effects on neutrophil migration.
We found that the entire pPI, as labeled by Chx1 expression, contained two groups of Dac+ and dimm-GFP-expressing cells, the larger and posterior of which were of the IPC NB lineage, as previously shown by clonal analysis (Wang et al., 2007) (see Fig. S3A in the supplementary material).
For this reason, we used the human prostate cancer cell line PC3 labeled by stable expression of luciferase gene (PC3/luc) that was previously applied in other similar in-vivo models [14].
In all cases, the posterior compartment is labeled by GFP expression (green).
The sensitivity and specificity of our sorting scheme were confirmed by using a mouse strain in which SCs and their progeny were genetically labeled by YFP expression.
Animals injected with fst dsRNA developed aberrant brains as labeled by chat expression, and in some cases produced no blastemas (n = 7/10 aberrant, 1/10 no blastema), while animals injected with control dsRNA regenerated normally (10/10 normal).
While in control and dominant negative expression experiments, single RGCs were labeled (upper panel), in the Ntf3 overexpression situation, single membrane bound eGFP (eGFPCaax) labeled RGCs were growing into a tectum overexpressing Ntf3 (labeled by RFP expression, shown in magenta, lower panel).
