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However due to the overlap between the transcripts, we were not able to determine if sites labelled by both probes expressed both AmphiGli-L and AmphiGli-S, or just expressed AmphiGli-L.
Tip cell MVs labelled by both CD31 and CD105 have an estimated mean diameter of 717.02 ± 130.01 nm (743.64 ± 138.93 at 9 10 wg and 702.12 ± 125.16 at 22 wg) measured on single optical planes from projection stacks at suitable intervals, as shown in the animation (Online Resource 2).
In SoFa1, all RGCs are labelled with gapRFP and therefore show a red membrane labelling; all inhibitory neurons are labelled by both gapRFP and cytGFP, and thus show a red membrane and a green cytoplasmic labelling; all BCs are labelled with gapCFP, so all show blue membrane labelling.
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As shown in Fig. 5A, PKD was labeled by both BirA*-PI4KIIα and BirA*-PI4KIIIβ; this finding is consistent with previous data showing that PI4KIIIβ is a substrate of PKD (Hausser et al. 2005).
For comparison, neurons that were labeled by both TH and DAT antibodies, and non-immunoreactive tissue within the VTA, were also collected and analyzed by PCR.
On average, 80 100% of intracellular bacteria were labeled by both LAMP-1 and LAMP-2 (Fig. 1B) suggesting that both proteins are prominently associated with the SCV and Sifs.
Single-Cys variants S47C and S313C were >70% labeled by both AF488 and TR.
A self hybridisation (reference labeled by both Cy5 and Cy3) was carried out in another array.
In specimens displaying SNAI1 nuclear positivity the same cell population was labeled by both antibodies.
Of these, 511 proteins were labeled by both the light and heavy isoform of ICAT, in both biological replicates.
Since the astroglial cells were labeled by both dyes, they appeared as yellow cells, while neurons were labeled by green OGB1-AM only.
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