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Plotted data were obtained by scanning and quantifying of labelled bands.
In particular this method is similar to that described in Bodnarenko and Chalupa [3] and the only difference is that we have used ChAT immunostaining to obtain two labelled bands corresponding to the projections of cholinergic amacrine cells.
While bistratified RGCs present a double-layered segregated arborisation with respect to the two anti-ChAT labelled bands (Fig. 1C and F), monostratified RGCs have dendrites restricted to the ChAT positive band inside sublamina b (Fig. 1D and G) or around the outermost ChAT positive band in sublamina a (Fig. 1E and H).
Labelled bands were identified using enhanced chemo-luminescence (Amersham).
The labelled bands were detected using Amersham ECL western blotting system according to manufacturer's specifications.
The membrane was then exposed to the appropriate horseradish peroxidase-conjugate secondary antibody (1 : 5000) (Sigma) for 1 h at room temperature and labelled bands were detected with a chemiluminescence commercial kit (Immun-Star™, Bio-RadHercules, CA, USA).
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A variety of "microgenres" have subsequently emerged, often labeling bands according to traits that deviate from regular grindcore, including goregrind, focused on themes of gore, and pornogrind, fixated on pornographic lyrical themes.
The labeled bands were visualized using chemiluminescence (Pierce, Rockford, IL).
Only unambiguously and intensely labeled bands were scored.
The 1D4 antibody predominantly labeled bands around 40k (arrowhead in Fig. 1A), which are most likely PrUV, PrB and PrV opsins.
The identity of 59Fe labeled bands as ferritin and Tf was established by immunoblotting under native conditions as described previously [13].
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