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Soils were incubated in 14CO2 labelled atmosphere for 90 days at three CO2 concentrations: 0.04%, 0.4% and 4%.
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After incubation for 24 72 h at 37 °C in a 5% CO2 atmosphere, the labelled A431 cells, that had invaded through the Matrigel, were scanned by the bottom-reading type fluorescence plate reader (Labsystems Fluoroskan Ascent, Thermo Electron Co., Waltham, MA, USA) at 544/590 nm (absorption/emission).
One was labelled "nurse", the other – inadvertently pressed – labelled "nukes".
The labelled 7D12 (300 μg) was incubated at 37°C in a CO2-enriched atmosphere (5% CO2) with freshly prepared human serum (1 4) in the presence of 0.02% NaN3.
For this study, C-labelled plants grown under controlled conditions in an Experimental Soil Plant Atmosphere System (ESPAS; IsoLife BV, Wageningen, The Netherlands) (Gorissen et al. 1996) were used.
All labelled Indian agents.
Labelled as non-core.
These were labelled "neighborhood planning areas".
But atmosphere?
The atmosphere.
Nice atmosphere.
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