An alternative, simpler design would be to use Cy5-labeled probes labelled at the 3' end instead.
The primary sequence numbers of H. sapiens cGAS are labelled at the top of the line.
Pyrene labelled at the 5′ end of oligonucleotide segment could be easily trapped into the cavity of β-CDP via host-guest interaction, leading to a significant enhancement of fluorescence intensity/anisotropy.
Four distinctive framings emerged from this analysis, labelled: 'At the very least we need more research'; 'We are the planetary maintenance engineers'; 'Geoengineering is a political project'; and 'Let's focus on Carbon.' Results indicate a strong polarity around divergently construed pros and cons of geoengineering as a whole – underscoring the political salience of this term.
As for the grand finale, the audience seemed largely gratified, though far from staggered, when the top gong was handed to "The Shape of Water" — or, as it was labelled at the foot of the screen, "Shape of Water," which presumably heralds an industry-wide crackdown on definite articles.
Yellow signs are labelled at the intersection of every trail on the TMB.
The forward primer was labelled at the 5'end with the dye 6FAM.
Both oligonucleotides had been labelled at the 5'-end with [γ-32P] and T4-polynucleotidekinase (NEB, USA).
All the probes were labelled at the 5' end with 6-carboxy fluorescein (FAM) and the 3' end with 6-carboxy-tetrametil rhodamine (TAMRA).
The probes were oligonucleotides synthesized on the basis of sequences complementary to the corresponding miR (see Table 1) and labelled at the 5'end with 32P-γATP.
Both control probes were labelled at the 5'end with either the indocyanine dye Cy3, showing red fluorescence, or with FITC.
