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Addressing a news conference, Ospreys director of coaching Johnson added: "That can have unfair accusations labelled against people.
SoMs have been labelled with a green circle and the scores for the top three predictions are labelled against the atom: green text is used where a SoM is identified, red text is used with a corresponding red ring where a non-SoM is identified.
The former value was significantly greater than that of the specificity control (0.12, HeLa cells labelled against phospho-ERK1/2 plus both secondary antibodies), and both interactions were significantly greater than in the negative control (Fig. 5, HeLa cells labelled against phospho-ERK1/2 plus Cy3).
An additional control for the specificity of the interaction was performed with cells transfected with GFP and further labelled against histones or VDAC and the Cy3 secondary antibody as above.
To detect HTLV-1 Gag matrix protein in infected cells the samples were labelled against HTLV-1 Gag p19 [33] using the DAB kit from Molecular Probes (Invitrogen Ltd, Paisley, UK ).
Isolated mitochondria were labelled with MitoTracker Deep Red (Invitrogen) (100 nM, 45 min at 37°C), fixed in 4% paraformaldehyde, permeabilized and labelled against phospho-ERK1/2 and a secondary antibody conjugated to Cy3 as above.
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(There are evolutionary biologists who protest against the simple "Darwinism" label, against "branding" it like a single-barrel Bourbon, but movement names tend to be taken, not chosen).
Human epidermis (Patient One) labeled against calmodulin using immunofluorescence labeling with confocal microscopy imaging.
Human epidermis (Patient Two) labeled against phospho-MEK-1/2 (pS218/pS222) using immunofluorescence labeling with confocal microscopy imaging.
Human epidermis (Patient One) labeled against MEK-1/2 using immunofluorescence labeling with confocal microscopy imaging.
Human epidermis (Patient One) labeled against phospho-ERK-1/2 (pT183/pY185) using immunofluorescence labeling with confocal microscopy imaging.
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