The proteins were then detected by enhanced chemiluminescence (ECL+, Amersham) after labeling with a horseradish peroxidase-labeled secondary antibody according to the manufacturer's instructions (GE Healthcare, Chalfont St. Giles, Great Britain).
More recently, NimbleGen introduced a new labeling method based on double-stranded cDNA synthesis followed by labeling with a DNA polymerase by extension of 5'-labeled random primers [ 6].
The French tried to claim him as an existentialist and others described him as an old-fashioned stoic, but he would have dismissed such labeling with a sardonic wisecrack.
After labeling with a radionuclide, functionalized nanostructures can serve as good candidates for molecular imaging probes (PET or SPECT).
After the incubation, cells were counted using fluorescent labeling with a CyQuant Cell Proliferation Assay Kit (Biosciences).
Selective protein labeling with a small molecular probe is a versatile method for elucidating protein functions under live-cell conditions.
Dual fluorescence labeling with a specific mouse monoclonal antibody against LHβ demonstrates that KiSS-1 and GPR54 are expressed by the gonadotrophs.
Furthermore, we quantified intracellular metabolic fluxes by employing stable isotope 13C labeling with a mathematically designed substrate mixture, gas chromatography mass spectrometry, and comprehensive isotopomer balancing.
Properties such as high-efficiency intracellular labeling with a short incubation time, a relatively low dose of nanoparticles, and non-cytotoxicity make HRP@HSNs suitable for intracellular detection of ROS.
The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC MS/MS).
Protein labeling with a synthetic probe is a powerful method not only for elucidating protein function in live cells but also for providing new tools to explore complicated biological processes that are associated with medical diagnosis.
