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In this study, however, we observed rare double-labeled (CldU+ IdU+) cells within a short labeling period (14 days) regardless of genotype (Table S1).
The fact that EdU labeling was not usually coincident with cytoplasmic and nuclear accumulation of β-catenin is probably due to the fact that, whereas relocalization of β-catenin is a transient event, the EdU-labeled cells had undergone S-phase any time during the 12-hour labeling period.
Furthermore, as well as the rapid appearance time of labeled keratin on the skin surface, the keratin that was detected appeared to be nearly 100% newly synthesized during the labeling period.
After the labeling period, the medium was removed and the cells were washed with PBS twice and then incubated in complete medium for 30 min for efflux of any free FDG.
The ratios represent accumulated cell proliferation during each labeling period.
One cuvette enclosed a fully expanded mature leaf (leaf # 14 16), which was exposed to 13CO2 during the labeling period.
For SCD inhibition, CVT-11127 was added at the indicated doses to the growing media for 22 h prior the labeling period.
In contrast, age-matched Rip-Tag2 transgenic mice incorporated BrdU in more than half their β-cells during the same labeling period.
We found that in RPS9 knockdown cultures very little mature 18S rRNA was produced during the labeling period (Figure 4C), however overall cellular RNA synthesis continued (Figure 4D).
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Interestingly, we observed several colocalized BrdU-positive β-cell pairs or small clusters by confocal microscopy suggesting that these cells did undergo cell division in situ within the 3 day BrdU pulse-labeling period (Supporting Information Figure S6).
Instead, we observed that less Yor1-ΔF was synthesized in the initial 10-min pulse-labeling period when SOP4, a member of the EMC, was deleted.
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