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Southern blot hybridization of genomic DNA was performed using fluorescein-labeled probes (ECL Labeling Kit, Roche).
Membranes were hybridised with 32P-labelled DNA probe (DecaLabel DNA Labeling Kit, MBI Fermentas).
Single-stranded cDNA was fragmented and end-labeled with the GeneChip WT Terminal Labeling Kit (Affymetrix).
The probes were DIG-labeled using a kit (DIG-RNA labeling kit; Roche).
DNA probes were 32P-labelled using the Prime-It II DNA labeling kit (Stratagene).
Labeled cDNAs were synthesized with the FairPlay cDNA labeling kit (Stratagene, La Jolla, CA).
Both digoxigenin- and fluorescein-labeled antisense RNA probes were generated using an RNA labeling kit (Roche).
Alexa-488-labeled dSETDB1 antibody was made using Zenon rabbit IgG labeling kit (Molecular Probes).
Biotin-labeled RNA was subsequently synthesized using the GeneChip IVT Labeling Kit.
Digoxigenin-labeled RNA probes were prepared using an in vitro labeling kit (Roche).
Digitonin-labeled anti-sense RNA was produced using the Dig RNA labeling kit (Roche).
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