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At least for EM some effective methods are now available for establishing quantitative estimates of labeling distributions, and for determining more accurately how much of the labeling is due to the interactions of the antibody with the intended target.
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To examine whether the punctate reelin labeling was due to reelin bound to receptors, hippocampal neurons were treated with the Receptor Associated Protein fused with GST (GST-RAP).
This background information was used to determine whether a lack of labeling was due to missing or damaged cone outer segments.
Moreover, the 5-BP signal in the latter sections overlapped with anti-TG2 staining, suggesting that the 5-BP labeling was due to TG2 enzymatic activity (Figure 4D).
As additional controls, pre-immune sera were tested, excluding the possibility that the observed labeling was due to nonspecific reactivity of rabbit sera with leptospiral surface antigens (Fig. 3).
This suggests the variation in labeling was due to increased noise, rather than some sort of systematic variation.
Analysis of Irga6 and Irgb6 accumulation on to ME49 T. gondii strain vacuoles in double-labelled samples taken shortly after infection showed that the earliest increase in label is due to accumulation of Irgb6, followed several minutes later by Irga6 (Fig. 6C).
The label is due to pay Gaye's family 50% of the song's future songwriting and publishing royalties revenue.
'Before Today', their first album for the label is due on 6 June.
Whether the loss of label is due to degradation, dilution, or its dispersal to undetectable levels throughout the midgut tissues is not known.
To validate that the reduction in biotin label was due to proliferation-induced dilution of the label in HSCs, we next investigated how well the biotin dilution correlated with DNA replication.
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