Exact(10)
The probe contained a fluorescein fluorophore with long-wavelength response and an active epoxy labeling group.
A synthetic peptide contains the 6-aa C-intein (IC) followed by a small linker and the labeling group.
As a result, the C-terminus of the target protein will be joined through a peptide bond to the small linker followed by the labeling group, with the N-intein (followed by the affinity binder) and the C-intein excised.
They were the spliced protein ML (the target protein with the C-terminal labeling group) and the excised N-intein (the INC fragment), while the excised C-intein was too small (6 aa) to be seen on the gel.
Second, the 6-aa S11 C-intein is free of amino acid residues commonly used for chemical modification (Lys, Cys, Glu), therefore any one or a combination of these residues may be added after the C-intein in the synthetic peptide to serve as specific site(s) for chemical attachment of desired labeling group(s).
The labeling peptide IC-L was a 12-aa synthetic peptide having the sequence GVFVHNSAGSGK-L, in which GVFVHN was the C-intein, SAGSGK was the C-extein (or linker), and L was the labeling group (5-carboxy-fluorescein; λexc = 492 nm, λem = 517 nm) linked to the side chain of the lysine (K) residue of the C-extein.
Similar(50)
That attitude has applied across the board to treatment of individuals as well as in labeling groups.
Under this new assumption, labeling groups of neurons would still produce the diffuse labeling required for our models and observed in vivo.
Interscope Geffen A&M, the biggest label group of the Universal Music Group, said on Friday that the unit's co-chairman, Ted Field, had resigned.
"Moral disengagement" occurs when subordinates of a labeling group regard the interests of the labeled group as less relevant because of the political culture under which they live.
But in this case, even the label group is getting in on the act.
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Justyna Jupowicz-Kozak
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