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For the glucose labeling experiment, 1,814 acetylated peptides were significantly correlated between biological replicates.
For the acetate labeling experiment, 2,100 acetylated peptides were significantly correlated between replicates.
K502 of GDH displayed a large heavy acetylation incorporation half-life of 80.3 hours in the acetate labeling experiment.
These sites were detected in the glucose labeling experiment and displayed fast heavy acetylation half-lives comparatively to other acetyl sites.
On the other hand, only 126 out of 2,120 peptides from the acetate labeling experiment had a significantly increasing acetylation trend.
Moreover, we identified the interaction of 3b with VEGFR-2 in photoaffinity labeling experiment using HUVE cells.
Similar(8)
In earlier studies, an amide hydrogen/deuterium exchange pulse-labeling experiment detected a stable submillisecond intermediate that accumulates before the rate-limiting transition state.
To solve this problem, we further profiled cell cycle by BrdU-labeling experiment.
MD performed the immunostaining of the Rb family in human and mouse retinae and performed the [H]-thymidine pulse-labeling experiment.
To confirm that Roc-A inhibits p53 protein synthesis, we carried out a [S]methionine-metabolic pulse-labeling experiment and then immunoprecipitated p53 after Etoposide treatment.
To verify the accuracy and reliability of the above quantitative results, a reverse-labeling experiment was performed in which different concentrations of intermediate-labeled internal standard peptides were incorporated into the light labeled islet sample.
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