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And comparing the annotation result with Huang and Chen (2011) and Zhou et al. (2014) cited in Section 2.2, our labeling distribution is much more balanced as shown in Fig. 8.
Once this fraction-specific is known, it is straightforward to correct the initial labeling distribution to yield a specific labeling distribution profile.
Statistical analysis of the immunogold labeling distribution was performed by χ test according to published methods.
The numerous cysteine residues in the wild-type CaMKII sequence also introduce an uncertainty in the labeling distribution.
The technique provides a rapid, robust, and sensitive means of determining the fluorophore labeling distribution while also providing details of encapsulated fluorophore properties, including brightness and photostability.
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New methods have been developed to evaluate labeling distributions and labeling intensities by statistical means.
Finally, we describe how quantitative EM methods can be used to identify reproducible patterns of antibody labeling and also extract specific labeling distributions.
The theoretical approach nonetheless is general and applicable also to systems with complex binding stoichiometries, complex labeling distributions, and fluorophore quenching, where brightness changes occur upon binding.
At least for EM some effective methods are now available for establishing quantitative estimates of labeling distributions, and for determining more accurately how much of the labeling is due to the interactions of the antibody with the intended target.
To confirm that prothrombin cleavage was responsible for the altered label distribution, we investigated the binding of OG488-active site-labeled thrombin.
"Heathen" is the first album from Mr. Bowie's own recording company, Iso, which has major-label distribution through Sony.
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