Exact(8)
After washing with PBS, a saturated labeling condition was used for labeling: FITC-anti-His6 tag mAb (33 μg/mL, abcam) and cypate-conjugated hapten (Cy-Him, 1 μM) were added for His6-tag and anti-hapten scFv labeling, respectively, and incubated for 30 min at room temperature.
The high labeling condition provided the most striking imaging quality particularly for kinetic tracking.
In contrast, the low labeling condition had modest effects on virus activity in the liver and only small effects on viral genomes in the tested organs which did not appear to have perturbed relative distribution.
Normally, the products from the first two runs of labeling reaction on microchip were discarded in order to establish the optimal labeling condition.
To determine the optimal labeling condition, 3 × 10 PDB-MSCs were treated with different concentrations of C-TdR (PerkinElmer, Boston) (0.2μCi, 1μCi, 5μCi) and incubated for 72 h at 37°C with 5%% CO2.
Different fields of view (>3 regions) were analyzed on the microscope for each labeling condition, and representative results are shown.
Similar(52)
Sequence attributes currently defined in SO are for labeling conditions that are either present or absent.
The conditions of the rules generated include the conditions from the recommendations (labelled "(condition)" below) and additional conditions.
The "no label" condition was associated with the lowest average of correct choices (20.2 of 28 pairs).
The energy percent from carbohydrates was lowest in the CGDA-condition and highest in the "no label" condition (see Additional file 2).
Here we present optimized labelling conditions of a DOTA-peptide with an 225Ac/213Bi generator (< 222 MBq) for preclinical applications using DOTA-Tyr3-octreotate (DOTATATE), a somatostatin analogue.
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