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Cisplatin-induced cell death in a dose-dependent manner (lower levels of green labeling areas), as revealed by the micrographs shown in Figure 1a.
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Six days after initial wet-up, the labeled areas and their control counterparts were sampled and the soil kept at −20 °C prior to SIP processing.
Palestinian textbooks, used in Palestinian schools, as well as in Jerusalem municipality-administered schools in East Jerusalem, inconsistently defined the 1967 borders and failed to label areas and cities with both Arabic and Hebrew names.
Post mortem autoradiography and histological analysis of heart sections showed that the labeled areas coincided with fibrosis.
Large fluorescently labeled areas are discernable, with dark domains in between, where the perylene fluorophore is excluded.
Since the intensity of TO-PRO-3 fluorescence reflects DNA concentration [34], [35], the intensely labeled areas presumably correspond to heterochromatin, which is more condensed than euchromatin.
However, not all sections were scored when mapping heavily labeled areas.
At least 1000 tumour cell nuclei were examined in the most evenly and distinctly labelled areas.
The 4 repeat injections in area 10 showed similar labeling connectivity profiles in terms of the identity of labeled areas and the FLNe values for each projection.
The data were therefore analysed assuming that the cells in well labelled areas were representative of the total cell population in the neoplasm.
This procedure allowed separation of the intracranial space from the skull and nonbrain structures; however, this method required verification and manual detachment of incorrectly labelled areas before performing volumetry with the built-in "Measurevol" module.
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