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Horizontal brain sections from untreated mice, incubated with streptavidin-peroxidase alone, showed no labeling above background (Figure 5A).
Sequential images were recombined to create a single image incorporating all of the endothelial cell or smooth muscle cell labeling, above or below the focal plane of the internal elastic lamina (IEL), respectively.
However, KaiCTae does not show labeling above background by this assay when the samples are heated before loading prior to SDS-PAGE (Fig. 4A and left panel of Fig. 4B).
The control proteins KaiCaaa and KaiCTaa show very low labeling with 32P, while the control protein KaiCaae has labeling above background (presumably residues other than 426, 431, and 432 can sometimes phosphorylate).
Labeling intensity was recorded as 0 (no labeling), 1+ (weak labeling above background), 2+ (moderate labeling), or 3+ (strong labeling) as previously described [ 17].
mGat4 localized predominantly to glial cells, consistent with previous reports (Borden, 1996), although faint labeling above background was also detected in a substantial fraction (80%) of DA neurons in SNc and lateral VTA (568 out of 713 Vmat2+ neurons).
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The network visualization above shows the connections between Alphabet's various investing groups and their respective portfolios.1 This graphic depicts 676 connections between six Google investing groups (labeled above in yellow), 570 portfolio organizations and 75 companies that acquired Alphabet-backed portfolio companies.
To probe the interaction between Aβ and ApoE CT; Aβ was labeled as above and ApoE CT was labeled using 3H1, directly conjugated to Cy3 [44].
Both dogs chose the labeled object above chance level in all conditions in which a familiar label was used.
The track was so incendiary that MCA refused to release it, so it was put out instead on the indie hip-hop label Up Above Records.
Brooks Brothers offers its own private label rendition, above, in mini-checks of steel-blue and black or a windowpane plaid in cream, light blue and gray ($128).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com