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This phenotype can be observed by staining the entire axon scaffold with anti-HRP antibodies or by labeling a subset of commissural axons using eg-GAL4 (Garbe et al., 2007; O'Donnell and Bashaw, 2013) in robo2, fra double mutants.
The predominant phenotype observed in our conditional DG knockout models of cerebellar dysplasia is widespread GC migration errors, corroborated by BrdU pulse-chase labeling; a subset of GCs expressing markers of mature neurons remain on the EGL surface, at sites of disrupted glia limitans and aberrant glial organization as well as reactive gliosis.
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The ground truth can be obtained either by manual identification (by a trained biologist or using software tools11) or from labelling a subset of the investigated cells with fluorescent stains (see Methods section).
This antibody labels a subset of differentiated taste bud cells in zebrafish, among other neuronal subtypes [46], [47], [48].
The chicken anti-PAP antibody labeled a subset of small- to medium-diameter neurons in rat DRG and labeled axon terminals in lamina II of the spinal cord (Figure 3A, E, I).
In this method, cell-type specific, but relatively low frequency, Cre-mediated recombination randomly labels a subset of neurons within the population of interest to generate an atlas that reveals representative soma locations, projections, and individual morphologies for all of the neurons of that class.
The authors state that Ac78c-GFP labels a subset of neuronal membranes.
The Golgi staining method labelled a subset of neurons in the hippocampus.
We labeled a subset of cells with a membrane-tethered GFP and examined whether their movement correlated with cuticle morphology.
The Group I marker Lysophosphatidic acid receptor 3 (labeledlabeled a subset of SNS-Cre/TdT+ neurons that did not overlap with Parv-Cre/TdT+ expression.
Dendritic spine quantification: to quantify spine density, Sema5A, Sema5B, Plxna1, and Plxna2 mice were crossed with Thy1-GFPm mice to genetically label a subset of postnatal hippocampal neurons.
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