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Quantification analyses showed that both antibodies have comparable penetration and labeling immunoreactivities, including the number of VACHT and VGLUT1-labeled vesicles per synaptic terminal, the labeling density and the percentage of vesicles labeled within a terminal.
Meanwhile, sub-loess and sub-paleosol units have been identified and labeled within loess and paleosol units.
The subsequent use of three different peroxidase substrates enabled us to unequivocally detect structures that were labeled within a three-color paradigm.
The use of purified mKate2-FKBP allowed most of the VAMP2-TagBFP-FRB (T2098L) on the cell surfaces to be labeled within tens of seconds (t1/2 of 13 s) with very high specificity.
At lower concentrations of the FKBP-AP21967 complex (50 nmol/L and 100 nmol/L, Fig. 1E), several minutes are required for the labeling of most of the target cell surface proteins, whereas at the higher concentration of 1 μmol/L, the majority of the VAMP2-TagRFP-FRB (90%) was labeled within 1 min (Fig. 1E).
We observed a significant difference between the gt/gt and wt cell lines in the proportion of prophase/prophase-like cells that were labeled within this period.
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Brightfield images of the BrdU-labelled cells (147 197 total cells, about 30% labelled) within the time-lapse recorded fields were collected.
We observed NTS labeling within sparse epithelial cells of ducts and lobules (Figure 1B, 1 and 4).
In each case there was also labelling within the superior and inferior colliculi.
We quantified dpERK labeling within PI-marked muscle nuclei (Fig. 4D).
In the same specimen, a second glial cell is labelled within the VNC.
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