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The length of the bars labeled with orange shows the extent of gene expression.
Therefore, the cell nuclei of HeLa.S-Fucci cells during the cell cycle are labeled with orange of mKO2 fused to the ubiquitination domain of Cdt1 in G1 phase and green of mAG1 fused to the ubiquitination domain of geminin in S, G2, and M phases.
The probes were labeled with Orange or Green dUTP (Abbott Molecular, Abbott Park, Illinois, USA) by nick translation and cell nuclei were counterstained with DAPI (1 μg/ml) before visualization on Zeiss Axioplan epifluorescence microscope equipped with a SPOT RT3 CCD camera.
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RNA FISH was performed as reported (42) using a pool of six different 2.5 kb intronic regions of Satb2 amplified from BAC RP24-241H21 labeled with Orange-dUTP (Enzo Life Sciences).
Oh, and all flights carrying life-sustaining Wifi are clearly labeled with nice orange icons.
(C) Three-dimensional confocal projection of HeLa cells transiently transfected with the plasmid pECFP-Endo (encoding an endosome-specific reporter) and labeled with QD orange as above.[32].
Probes were labeled with Spectrum Orange or Spectrum Green by Nick Translation according to the manufacturers instructions (Abbot Molecular), followed by ethanol precipitation and resuspension in 0.1 ml of Hybrisol VII (MP Biomedicals).
BAC DNA was labeled with Spectrum Orange using a nick translation kit (Abbott Molecular).
For ECM degradation and cell colocalization analysis U937 cells were labeled with CellTracker Orange.
RP11-783B20 was labeled with spectrum orange by nick translation (Abbot Laboratories) according to the manufacturer's instructions.
Chromosome 7 was labeled with Spectrum Orange (Abbott Laboratories; Chicago, IL) and chromosome 13 was labeled with Dy505 (Dyomics; Jena, Germany).
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