Sentence examples for labeled with light from inspiring English sources
106 MCF-7 cells labeled with heavy isotope were then counted by flow cytometry, and mixed with equal amounts of cells labeled with light isotope.
Next, the peptides from CSF of animals after sham, moderate, and severe injury were labeled with light (CH2O), medium (CD2O), and heavy (13CD2O) isotopologues of formaldehyde, respectively [20].
Cells labeled with light isotopes were then treated with LPS/IFN-γ for 18 h, while cells labeled with heavy isotopes were used as controls.
To test whether our method does preserve accuracy, the same concentration of proteins extracted from RAW264.7 cells labeled with light and heavy isotopes were both treated with 1 mM S-nitrosoglutathione (GSNO) in the dark for 1 h.
Control samples were labeled with light 12C acrylamide isotope (7.1 mg/mg protein) (>99.5% purity, Fluka), and experimental samples were labeled with the heavy 13C-acrylamide isotope (Cambridge Isotope Laboratories, 7.4 mg/mg protein) for 1 h at room temperature.
hESC lysate for each time point (6 mg) was digested, followed by stable isotope dimethyl labeling, whereby the non-stimulated hESC (0 min) was labeled with light dimethyl labels, the 5 min time point with intermediate labels, and the 15 min time point with heavy dimethyl labels.
At some point during growth or sample preparation, proteins or peptides from each sample are labeled with either "light" or "heavy" forms of a chemical tag.
The control sample was labeled with the light, the specific elution sample with heavy cICAT label (Applied Biosystems, Foster City, CA, USA).
Samples collected from mock transfected cells labeled with the light (d0) isotope and those collected from Flag-M2 transfected cells labeled with the heavy (d6) isotope.
Different cell populations are grown in medium containing amino acids labeled with stable light or heavy isotopes.
Therefore in dSS-1, all RSH + RSNO groups were labeled with the light isotopologue (NEM) and all SS groups were labeled with the heavy isotopologue (d5-NEM).
