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Fluorescent peptide KSp21 labeled with dark quencher QSY21 and Chromis-645-A-PDA fluorescent dye (50 mg) was synthesized by Cambridge Research Biochemicals CRBB).

Importantly, the most strongly contacted cells in this group (labeled with darker reds in Figure 5H) have relatively high neurogranin expression.

Moss, whose pale mien and angular skinny frame broke with the super-charged, Glamazon glow of the supermodel pack that had been reigning the runways, was also labeled with a darker sticker − 'heroin chic'.

The length of the bars labeled with blue and dark yellow in Fig 3 represents the quantitation of high-confidence mapped tags and all mapped tags, respectively.

The MscL pentamer protein (light blue), centered at O, is labeled with two probes (dark blue) on two cysteine residues (specified by the residue #), while the other three residues (with the same residue # but on different subunits) remain empty / unlabeled (dashed green).

For illustration purpose, we set up the general scene in Figure 4 figure supplement 2. The pentameric MscL channel (light blue), centered at point O, is labeled with two probes (dark blue) on two cysteine residues (specified by residue #), while the other three residues (with the same residue # but on different subunits) remain empty/unlabeled (dashed green).

Cells labelled with DAB (dark brown) and cresyl (blue) were counted separately with Image-Pro Plusoftwarere.

The principal cell types in the mouse SC labeled with Otx2 antibody were dark or light round to oval cells.

An amount (50 μg) of proteins were labeled with 400 pmol of dyes (dark; on ice; 30 min) and then quenched (10 min) with 1 μl of 10 mM lysine.

The 147-bp DNA fragment 0A0 was internally labeled with the fluorescent dye AF488 and the dark quencher BHQ1.

The samples were rinsed with PBS and then incubated in the dark with second antibody labeled with the corresponding fluorescein moiety at 37°C for 2 h.