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To quantify the levels of the reference GAPDH mRNA, a target-specific primer-probe set (VIC labeled) was used (Human Endogenous Controls, catalog number: 4310884E, Applied Biosystems).
To quantify CXCR4 mRNA levels, a commercially available primer-probe set (FAM labeled) was used (TaqMan Gene Expession Assays, HS00237052m1, Applied Biosystems).
A diluted Fn solution (50 μg/mL, 10% FRET labeled) was used for incubation to ensure that only intramolecular FRET was measured to assess conformation of single Fn molecules.
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MGB probes hybridizing to the fully methylated (VIC labeled) and the fully unmethylated sequences (FAM labeled) were used.
A control probe of Cy5 labelled was used as a reference for data normalization.
Fluorescent labeling was used for all detections.
Reverse labeling was used as control.
According to Patten "The label was used to denigrate our work.
In Buckley v. Valeo, however, the label was used by the Court to signal a team effort, of sorts.
BrdU labeling was used to evaluate DNA synthesis.
Imagine if the "terrorism" label was used in cases of White Christian men.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com