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The cell permeant DNA stain Hoechst™ 33342 ("Hoechst") was included in the media, as an orthogonal reference marker which labeled the cell nuclei to allow segmenting the individual cells in the images [27, 28].
To analyze changes in the morphology of individual muscle cells during tail extension we labeled the cell membranes with a plasma membrane marker to allow identification of cell boundaries and therefore provide information on cell morphology and topology [1].
SUR1 antibodies also strongly labeled the cell surface (Fig 9C).
As with FM 4-64FX, Celabeledlabeled the cell surface as well as surface-connected IPMCs.
PH-GFP labeled the cell surface as well as IPMCs, indicating that PI 4,5 P2 is abundant in these locations.
For this purpose, we labeled the cell membrane using PKH26, and evaluated the mean fluorescence intensity at the time of staining and 48 h after the last dose of radiation by flow cytometry.
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But Dr. Frisen realized several years ago that nuclear weapons tested in the atmosphere until 1963 had in fact labeled the cells of the entire world's population.
They identified and chemically labeled the cells in the animals' brains where that memory was being formed.
To find out what was interfering with proper cell division, the team fluorescently labeled the cell-division machinery in cloned rhesus embryos.
Moreover, we labeled the cells of the patients diagnosed with HER-2+ epithelial ovarian cancers (Fig. 2b).
Once labeled, the cells were examined under an Olympus IX71 fluorescence microscope.
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