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Inset the labeled spots represent CIE coordinates at different luminances.
In BeWo cells, the fluorescent NBD-cholesterol analog indeed brightly labeled spots corresponding to lipid droplets, and to a lesser extent the ER (not shown).
Labeled spots were detected with a Typhoon 9400 Variable Mode Imager (GE Healthcare) and digital image analysis was conducted with SameSpot Progenesis (Nonlinear Dynamics).
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Subsequently, the CyDye3 and CyDye5 labeled spot volumes were divided by the spot volume of the corresponding protein spot in the internal standard (CyDye2) image.
"These 99 per centers are stealth softies who are happy to wear concealer just as long as it's labelled "spot cream", and to carry a purse only if it's billed as a messenger bag".
There were four distinct protein spots labelled as spots 1 4.
The labeled protein spots in the gel were visualized with Typhoon Trio variable imager (GE Healthcare).
Following this, hearts were dissected and, in many instances, immunostained for labeled twin spots (GFP and Myc).
The labeled protein spots were subjected to in-gel trypsin digestion after de-staining the gel plugs.
Lysates from the cells were separated by two-dimensional (2D) gel electrophoresis, and labeled protein spots were analyzed by mass spectrometry.
Intracellular TAT-SA, visualized as small, intensely labeled, grainy spots was localized in large intracellular vesicles near the cell surface or in endocytic-like vesicles adjacent to the nuclear membrane at 4 h p.t (Fig. 2A).
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