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The condition, which Gerbasi et al labeled "species identity disorder", had a physiological component too, with many reporting experiencing phantom body parts, like tails or wings.
An enhancement in the rate of mass transport through such membranes has been reported for porous alumina with the use of fluorescently labeled species [20].
Single labeled species were separated by C18 reversed phase HPLC and analyzed by MALDI MS. FP assays were essentially carried out and analyzed as described [73].
Stability of the GFP plasmid varied among the labeled species and strains, with the most stable GFP label observed in E. coli O157 H7.
For example, it is almost impossible to avoid donor-only (d-only) labeled species in mixture with donor and acceptor (DA) labeled species.
However some cells displayed an additional PY labeled species within the nucleus.
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FISH, SC-FISH, and Fiber-FISH experiments using biotin and digoxigenin labeled species-specific ITS and 5S rDNA probes were performed following the methods published elsewhere [ 13, 18].
To verify the strict exclusion of multivalent formation or pairing between non-homologous chromosomes, we performed a cytological analysis of the resulting hybrids during the transition from meiotic metaphase I to anaphase I. Chromosomes from different parental origin were distinguished by fluorescence in situ hybridization with differentially labeled species-specific centromeric repeat probes.
Oligomers are thus distinguished from monomers by the presence in both detection channels of simultaneous fluorescent signals, the intensity of which can be used to estimate the oligomerization order of the dual-labeled species (by comparison with the signal emitted by singly labeled monomeric species).
WT V154C gave a mixture of labeled and unlabeled species, and suppression of BIM reduced the IASD-labeled species, although this effect was only evident following ABT-737 treatment.
The technique principally involves quantitative measurement of two ions that differ by two mass-to-charge ratio (m/z) units, one for the 11C-labeled species, denoted [11C]species, and another for the carrier, denoted [M + 1]carrier.
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