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The results were quite similar with the mouse Nxnl1 construct −351/+51 (Figure 5B), but by examining more extensively the labeled slices, we could identify some GFP positive cells in the inner part of the retina as represented in the merged portion of the panel.
The labeled slices were mounted and exposed to a super-sensitive phosphor screen (PerkinElmer, USA) at room temperature for 8 h.
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The most densely labeled slice in a series of 80-micron sections was selected (the presumed site of injection) and the intensity of all cell somata in the selected slice was quantified.
The "gold standard" for tissue diagnostics is the histological examination, which is performed by means of white light optical microscopy on cryosectioned, processed, and labelled slices of tissue.
The images obtained from four different slices of single aliquots (labeled as Slice 1, Slice 2, Slice 3, and Slice 4), show contrast enhancement as the iron concentration increases.
The selected MR images that correspond to the sectioning of the atria are labeled as slices numbering from 1 to 3, starting from top of the heart down to its apex through an axis oblique to the direction from head to foot, for examination and flow analyzes.
As a demonstration of this, Kinoshita et al. [ 14] labeled tectal slices of adult rainbow trout with a voltage-sensitive dye and imaged the propagation of activity as current was applied to the anterior pole of the SO and SFGS.
For double labelling, slices were postfixated in 4% paraformaldehyde (PFA) and incubated in blocking solution.
It is up to you to label your slices.
Afterwards, we labelled the slices with antibody to myelin basic protein to define the white matter and with DAPI to define cell nuclei, and quantified the percentage of cells which were dead (i.e. took up propidium iodide into their soma) in the white matter and the grey matter.
To label microglia, slices were incubated for 20 min in 1 mL of external solution containing isolectin-B4 conjugated to Alexa 488 (Molecular Probes, UK: excited at 475 nm and emission collected at 535 nm) at a concentration of 50 μg/mL (Streit & Kreutzberg, 1987).
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