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The counts are made in series of scans, which are made across the labeled section with a random start (SURS; Fig. 3).
Thus the concordance of the assessments improved, simply by making the appropriate revision, that is, when staging IHC labeled section, the emphasis should be on the labeled NTs rather than on NFT.
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All 60 tissue labeled sections was positive.
All labeled sections were mounted in Mowiol embedding medium.
The labeled sections were observed under a Leica compound microscope, equipped with fluorescent lamp and GFP filter.
The original Talairach atlas was published in print and labeled sections from a single hemisphere of the cadaver of a 60-year old woman.
Images from silver-enhanced nanogold labeled sections were captured on an Axiophot-2 visible/fluorescence microscope with 100X optical lens using AxioVision4Ac software system (Carl Zeiss, Germany).
The number of SMI32-positive projection neurons bearing a PNN was determined on double labeled sections with SMI32 antibodies and WFA histochemistry.
Next we double labeled sections throughout chimeric regions of each animal for both ChAT and ubiquitin, one marker of degenerating motor neurons in this model [19].
Images of the granule cell layer (GCL) and CA1 of MeCP2 labeled sections were traced digitally and intensity levels were analyzed with MetaMorph software (Molecular Devices).
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