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The idea is to be able to elicit a "truthful" response on sensitive feature(s) from the sampled respondents (of a finite labeled population of respondents), so that eventually the population mean of the sensitive feature can be unbiasedly estimated.
Antibody treated and untreated KO mice were injected with BrdU for 4 days, then subsequently CD8+ T cells response in the BrdU labeled population was measured at day 6 and 14 p.i. (Figure 3A).
Because neurites are packed so densely in neuropil (∼10 40 neurites per voxel of a typical diffraction limited microscope), for a densely labeled population of neurons the fluorescence from all such neurites tends to blend together, making individual neurites indiscernible.
The mouse studies reported thus far have not applied genetic labeling technologies to the morphologic analysis of neurons with large and complex arbors, an application that requires extremely sparse labeling and that is only rendered practical by limiting the labeled population to one or a few predetermined neuronal subtypes.
In mammals, genetically-directed cell labeling technologies have not yet been applied to the morphologic analysis of neurons with very large and complex arbors, an application that requires extremely sparse labeling and that is only rendered practical by limiting the labeled population to one or a few predetermined neuronal subtypes.
2) Figure 2: The clonal nature of the labeled population is crucial for the interpretation of the data.
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In the second method, we labeled populations of excitatory neurons based on projection target by using herpes simplex virus 1 (HSV1), which is efficiently taken up by axons and transported retrogradely.
Consistent with this, the labeled populations of layer 2/3 neurons studied here had extensive projections to infragranular layers.
Because many of the labeled populations of neurons overlapped in space, it is difficult to discriminate between populations based on location alone.
Usually a ratio of intensities is calculated for each probe or gene, corresponding to two different labeled populations of reverse-transcribed mRNA.
Our viral tracing method may allow trans-synaptic spread between dopamine neurons, which would make the trans-synaptically labeled populations appear more similar.
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